T7 RNA polymerase - Wikipedia

 

t7 rna polymerase application

T7 RNA Polymerase is a DNA-dependent RNA polymerase that has a high specificity for bacteriophage T7 promoter sequences. The enzyme synthesizes large quantities of RNA from DNA inserted into a transcription vector downstream from a T7 promoter. A T7 RNA Polymerase technical bulletin is . The second contains the T7 RNA polymerase gene under the control of a heat-inducible E. coli promoter. Upon heat induction, the T7 RNA polymerase is produced and initiates transcription on the expression vector, resulting in turn in the expression of the gene(s) under the control of p(T7).Cited by: In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different lipetusk.gasm: T7 phage.


T7 RNA Polymerase


Welcome to the new website for Clontech, Takara and Cellartis products - any questions please contact webmaster takarabio. The enzyme can incorporate labeled or unlabeled nucleoside triphosphates into an RNA transcript. The enzyme is supplied in a buffer of 20 mM potassium phosphate pH 7. The enzyme can incorporate labeled or unlabeled nucleotide triphosphates into an RNA transcript. Please refer to Cat. Our products are to be used for Research Use Only.

They may not be used for any other purpose, including, but not limited to, t7 rna polymerase application, use in humans, t7 rna polymerase application, therapeutic or diagnostic use, or commercial use of any kind.

Our products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a t7 rna polymerase application to third parties without our t7 rna polymerase application written approval. Chamberlin, M. Nature t7 rna polymerase application, —31 Characterization of T7-specific ribonucleic acid polymerase. General properties of the enzymatic reaction and the template specificity of the enzyme.

Davanloo, P. Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Schenborn, E. Nucleic Acids Res. Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. All Rights Reserved. All trademarks are the property of Takara Bio Inc. Certain trademarks may not be registered in all jurisdictions.

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Read our cookie policy. Contact Sales. T7 RNA Polymerase. Notice to purchaser Our products are to be used for Research Use Only. Documents You May Also Like. Applications In vitro transcription Source Recombinant E, t7 rna polymerase application. Additional product information Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Log in to enjoy additional benefits Why sign up for an account?

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T7 RNA Polymerase - Thermo Fisher Scientific

 

t7 rna polymerase application

 

T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits high specificity for the bacteriophage T7 promoter sequence 5'-TAATACGACTCACTATA-3'. The enzyme can incorporate labeled or unlabeled nucleoside triphosphates into an RNA transcript. T7 RNA polymerase is function tested in the SP6/T7 Transcription Kit (Cat. No. 10 ). The standard assay with µg of the corresponding template contained in the control DNA labeled with 6 mM DIGUTP mix gives sensitivity by direct spot-test a 1 pg incorporation in 20 min. In biotechnology applications, T7 RNA polymerase is commonly used to transcribe DNA that has been cloned into vectors that have two (different) phage promoters (e.g., T7 and T3, or T7 and SP6) in opposite orientation. RNA can be selectively synthesized from either strand of the insert DNA with the different lipetusk.gasm: T7 phage.